%0 Journal Article
%T Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature
%A Teresa San-Miguel
%A Pedro P¨Śrez-Berm¨˛dez and Isabel Gavidia
%J SpringerPlus
%D 2013
%I Springer
%R 10.1186/2193-1801-2-89
%X The protocol can be widely applied to proteins with a heterologous expression which was limited by loss of activity at high temperatures or by low soluble recombinant protein yield.
%K Early log phase
%K Functional proteins
%K Low temperature
%K Soluble recombinant proteins
%U http://www.springerplus.com/content/2/1/89/abstract