%0 Journal Article %T Cloning and identification of a novel human glioma differentiation related geneGDR1 %A Guangtao Li %A Hulin Jin %A Songnian Hu %A Jian Jin %A Chun Tu %A Jiangang Yuan %A Boqin Qiang %J Chinese Science Bulletin %@ 1861-9541 %D 2000 %I %R 10.1007/BF02884978 %X In order to identify the genes associated with glioblastoma differentiation, some ESTs, expressed differentially in the control cell and the differentiated human glioblastoma cell line BT-325 induced by the all-trans retinoid acid, have been isolated by the method of DDRT-PCR. Of the 46 ESTs sequenced, 19 are from new genes. A full-length 1 535-bp cDNA, termed geneGDR1, has been isolated from the human cDNA library using the probe designed according to one of the novel ESTs, HGBB098. The open reading frame ofGDR1 gene encodes a putative protein containing 334 amino acid residues. Blast against the current GenBank DNA and protein sequence database did not reveal significant homology with any known proteins. RT-PCR shows thatGDR1 mRNA level increased in the differentiated BT-325 cells after being treated with RA. The different expression patterns ofGDR1 mRNA in human tissues have been detected through the multiple tissue Northern blot hybridization. %K human glioma %K GDR1 %K RT-PCR %U http://link.springer.com/article/10.1007/BF02884978