%0 Journal Article
%T Cloning and identification of a novel human glioma differentiation related geneGDR1
%A Guangtao Li
%A Hulin Jin
%A Songnian Hu
%A Jian Jin
%A Chun Tu
%A Jiangang Yuan
%A Boqin Qiang
%J Chinese Science Bulletin
%@ 1861-9541
%D 2000
%I 
%R 10.1007/BF02884978
%X In order to identify the genes associated with glioblastoma differentiation, some ESTs, expressed differentially in the control cell and the differentiated human glioblastoma cell line BT-325 induced by the all-trans retinoid acid, have been isolated by the method of DDRT-PCR. Of the 46 ESTs sequenced, 19 are from new genes. A full-length 1 535-bp cDNA, termed geneGDR1, has been isolated from the human cDNA library using the probe designed according to one of the novel ESTs, HGBB098. The open reading frame ofGDR1 gene encodes a putative protein containing 334 amino acid residues. Blast against the current GenBank DNA and protein sequence database did not reveal significant homology with any known proteins. RT-PCR shows thatGDR1 mRNA level increased in the differentiated BT-325 cells after being treated with RA. The different expression patterns ofGDR1 mRNA in human tissues have been detected through the multiple tissue Northern blot hybridization.
%K human glioma
%K GDR1
%K RT-PCR
%U http://link.springer.com/article/10.1007/BF02884978