%0 Journal Article
%T PF18-3, a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes
%A Hong Wang
%A Yan Li
%A Xuewen Pang
%A Weifeng Chen
%J Chinese Science Bulletin
%@ 1861-9541
%D 2000
%I 
%R 10.1007/BF02909769
%X PF18-3 monoclonal antibody (mAb), one of the rat mAbs against mouse thymic stromal cells (MTSC), has been found to inhibit thymocyte apoptosis induced by a mouse thymic dendritic cell line, MTSC4, in previous co-culture study. The aim of this research is to investigate the character of PF18-3 rnAb recognized molecule (PF18-3 molecule) and its role in MTSC4-induced thymocyte apoptosis. The characterization of PF18-3 molecule expression has been conducted by FACS analysis. PF18-3 molecules have been found to express on MTSC4 as well as on Con A activated but not freshly isolated thyrnocytes. Up-regulated expression of PF18-3 molecules has been also observed on thymocytes after being co-cultured with MTSC4 for 48 h. The results from FACS analyses by PI staining for detecting apoptosis-related hypodiploid and by PF18-3 rnAb staining reveal that PF18-3 molecules expresss specifically on the apoptotic subgroup of thymocytes with high hypodiploid content. The PF18-3 molecule expressed on apoptotic thymocytes with 35 ku of molecular weight, identified by immunoprecipitation and western blotting, is thus likely to be a molecule involved in thymocyte apoptosis.
%K thymocytes
%K cell apoptosis
%K monoclonal antibody
%K mediator
%U http://link.springer.com/article/10.1007/BF02909769