%0 Journal Article
%T Establishment of Jurkat tet-on cell line
%A Hongwei Wang
%A Jing Zhang
%A Ninghua Wu
%A Yufei Shen
%J Chinese Science Bulletin
%@ 1861-9541
%D 2000
%I 
%R 10.1007/BF02887100
%X Tet-control system is developed to tightly control target gene expression in mammalian cells by using the regulatory elements of tetracycline-repressor of the transposor Tn10 fromE. coli. We have transfected reverse tetracycline-controlled transactivator gene (rtTA) into genome of Jurkat cells and established two Jurkat tet-on cell lines. Induction of luciferase reporter activity with doxycycline, a tetracycline derivative, is dose-dependent with a peak value of 32-fold increment. Establishment of Jurkat tet-on cell lines greatly facilitates quantitative studies on target gene functions in the cells.
%K tet-on cell line
%K Jurkat cell
%K tetracycline control system
%U http://link.springer.com/article/10.1007/BF02887100