%0 Journal Article %T Fragile X mental retardation protein interacts with TDG %A Yuting Chen %A B. Bardoni %A Ming Yu %A Ning Zhu %A Guanyun Wu %A J. L. Mandel %A Yan Shen %J Chinese Science Bulletin %@ 1861-9541 %D 2000 %I %R 10.1007/BF02887096 %X Fragile X syndrome is the most common form of inherited mental retardation disease, resulting from absent of expression of its disease geneFMR1. To study the function of the fragile X mental retardation protein (FMRP) through protein/protein interaction, a mouse embryo cDNA library was screened by the yeast two-hybrid system. A clone was found to interact specifically with FMRP. The cDNA of this clone (Genbank accession number af 102875) encoded a protein highly homologous to human G/T mismatch-specific DNA thymine glycosylase (hTDG). Interactions between various alternatively spliced FMRP isoforms and a series of mTDG deletion proteins were further studied in the yeast two-hybrid system and their interaction amino acid regions were determined. Interaction between FMRP and TDG existed inside exon 13 of FMRP (amino acid residue 397¨C425) and around amino acid residue 122¨C346 of TDG. These results will be helpful to the study of the biological role of FMRP. %K fragile X mental retardation protein %K G/T mismatch-specific DNA thymine glycosylase %K yeast two-hybrid %K protein/protein interaction %U http://link.springer.com/article/10.1007/BF02887096