%0 Journal Article
%T Fragile X mental retardation protein interacts with TDG
%A Yuting Chen
%A B. Bardoni
%A Ming Yu
%A Ning Zhu
%A Guanyun Wu
%A J. L. Mandel
%A Yan Shen
%J Chinese Science Bulletin
%@ 1861-9541
%D 2000
%I 
%R 10.1007/BF02887096
%X Fragile X syndrome is the most common form of inherited mental retardation disease, resulting from absent of expression of its disease geneFMR1. To study the function of the fragile X mental retardation protein (FMRP) through protein/protein interaction, a mouse embryo cDNA library was screened by the yeast two-hybrid system. A clone was found to interact specifically with FMRP. The cDNA of this clone (Genbank accession number af 102875) encoded a protein highly homologous to human G/T mismatch-specific DNA thymine glycosylase (hTDG). Interactions between various alternatively spliced FMRP isoforms and a series of mTDG deletion proteins were further studied in the yeast two-hybrid system and their interaction amino acid regions were determined. Interaction between FMRP and TDG existed inside exon 13 of FMRP (amino acid residue 397¨C425) and around amino acid residue 122¨C346 of TDG. These results will be helpful to the study of the biological role of FMRP.
%K fragile X mental retardation protein
%K G/T mismatch-specific DNA thymine glycosylase
%K yeast two-hybrid
%K protein/protein interaction
%U http://link.springer.com/article/10.1007/BF02887096