%0 Journal Article
%T RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C. elegans
%A WanJuan Feng
%A Tao Liang
%A JunWei Yu
%A Wei Zhou
%A YongDeng Zhang
%A ZhengXing Wu
%A Tao Xu
%J Science China Life Sciences
%@ 1869-1889
%D 2012
%I 
%R 10.1007/s11427-012-4296-9
%X The molecular mechanisms by which dense core vesicles (DCVs) translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis. RAB-27/AEX-6, but not RAB-3, was found to be required for peptide release from neurons. By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy, we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.
%K exocytosis
%K dense core vesicles
%K RAB-3
%K RAB-27
%K C. elegans
%K total internal reflection fluorescence microscopy
%U http://link.springer.com/article/10.1007/s11427-012-4296-9