%0 Journal Article
%T Stress influenced increase in phenolic content and radical scavenging capacity of Rhodotorula glutinis CCY 20-2-26
%A Raj Kumar Salar
%A Milan Certik
%A Vlasta Brezova
%A Marta Brlejova
%A Vladimira Hanusova
%A Em¨ªlia Breierov¨¢
%J 3 Biotech
%@ 2190-5738
%D 2013
%I Springer
%R 10.1007/s13205-012-0069-1
%X Rhodotorula glutinis CCY 20-2-26 when grown under controlled stress of either NaCl (1¨C5 %) or H2O2 (1¨C5 mM) on basal media exhibited a twofold increase in its total phenolic contents. The radical scavenging capacities (RSCs) as determined by ABTS test were found to be highest in 4 mM H2O2 (1.44 mM TEAC mg 1) and 4 % NaCl (1.13 mM TEAC mg 1) as compared to control samples (0.41 mM TEAC mg 1). Similarly, the RSCs as determined by DPPH test were also highest in 4 % NaCl (1.83 mM TEAC mg 1) and 4 mM H2O2 (1.78 mM TEAC mg 1) compared to control (0.48 TEAC mg 1). The relative RSCs from EPR spin-trapping assay for H2O2-stressed cultures were highest in 1 mM H2O2 (56.1 ¦ÌM TEAC g 1) whereas in NaCl-stressed cultures it was highest in 5 % NaCl (44.6 ¦ÌM TEAC g 1) as compared to control (30.9 ¦ÌM TEAC g 1). Five phenolic compounds (gallic acid, benzoic acid, catechin, caffeic acid and ferulic acid) were detected for the first time in R. glutinis CCY 20-2-26.
%K Rhodotorula
%K Phenolics
%K Radical scavenging capacity
%K HPLC
%K EPR/spin trapping
%U http://link.springer.com/article/10.1007/s13205-012-0069-1