%0 Journal Article
%T Cloning and prokaryotic expression ofHGLP cDNA
%A Zhou Haijun
%A Huang Xiaowei
%A Zhou Yan
%A Li Guangtao
%A Yin Bin
%A Ma Yanling
%A Peng Xiaozhong
%A Yuan Jiangang
%A Qiang Boqin
%J Chinese Science Bulletin
%@ 1861-9541
%D 2001
%I 
%R 10.1007/BF02900684
%X A novel cDNA,HGLP, which encodes a G-protein coupled receptor (GPCR) like protein, has been isolated and cloned. The coding region of the humanHGLP predicts a 7-transmembrane region protein with motifs of rhodopsin-like GPCR superfamily. Northern blot analysis reveals a 3-kb transcript in various human tissues examined. The N- and C-terminal coding regions ofHGLP, which are deduced as non-transmembrane regions, have been amplified by PCR and cloned into pET30a+ vector. Then the recombinant proteins are highly expressed inE. coli.
%K G-protein coupled receptor
%K HGLP
%K cDNA
%K prokaryotic expression
%U http://link.springer.com/article/10.1007/BF02900684