%0 Journal Article
%T Analysis of the ligand-binding domain of macrophage colony-stimulating receptor
%A Shouqing Luo
%A Dexian Zheng
%A Yanxin Liu
%A Qing Rao
%A Kefu Wu
%J Chinese Science Bulletin
%@ 1861-9541
%D 2000
%I 
%R 10.1007/BF02886077
%X RNAs have been extracted from human placenta. Extracellular regions of M-CSFR, D1-3, D2-3 and D3 motifs have been amplified with RT-PCR. The proteins have been expressed inE.Coli. Enzyme-linked immusorbent assay (EIA) shows that recombinant D1-3 possesses binding ability 3 times that of D2-3.Kd, the dissociation constant of the former is 11 nmol/L, and that of latter is 39 nmol/L. D3 lacks binding ability. These data suggest that D2-3 is the main site for M-CSF binding, D1 is an assistant site and also contributes to the conformation of site for ligand binding.
%K M-CSF
%K binding ability
%K structure-function
%U http://link.springer.com/article/10.1007/BF02886077