%0 Journal Article
%T Mitochondrial Ceramide-Rich Macrodomains Functionalize Bax upon Irradiation
%A Hyunmi Lee
%A Jimmy A. Rotolo
%A Judith Mesicek
%A Tuula Penate-Medina
%A Andreas Rimner
%A Wen-Chieh Liao
%A Xianglei Yin
%A Govind Ragupathi
%A Desiree Ehleiter
%A Erich Gulbins
%A Dayong Zhai
%A John C. Reed
%A Adriana Haimovitz-Friedman
%A Zvi Fuks
%A Richard Kolesnick
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0019783
%X Evidence indicates that Bax functions as a ˇ°lipidicˇ± pore to regulate mitochondrial outer membrane permeabilization (MOMP), the apoptosis commitment step, through unknown membrane elements. Here we show mitochondrial ceramide elevation facilitates MOMP-mediated cytochrome c release in HeLa cells by generating a previously-unrecognized mitochondrial ceramide-rich macrodomain (MCRM), which we visualize and isolate, into which Bax integrates.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019783