%0 Journal Article
%T Two-and Three-Layered Dissolving Microneedles for Transcutaneous Delivery of Model Vaccine Antigen in Rats
%A Asuka Ikejiri
%A Yukako Ito
%A Seishiro Naito
%A Kanji Takada
%J Journal of Biomaterials and Nanobiotechnology
%P 325-334
%@ 2158-7043
%D 2012
%I Scientific Research Publishing
%R 10.4236/jbnb.2012.33030
%X Purpose: Comparison of transcutaneous immunization of ovalbumin (OA) between two-and three-layered dissolving microneedles (MN) in rats. Methods: We prepared 500 ¦Ìm long two-layered and three-layered dissolving microneedle (2-MN and 3-MN, respectively) arrays from chondroitin sulfate as the base, and OA as the model antigen. The 2-MN containing OA at the acral portion and 3-MN with OA at the second portion were administered to rat skin transcutaneously. As a positive control, OA solution was injected subcutaneously (sc). The OA delivery and diffusion in the rat skin were studied using confocal microscopy with fluorescein-conjugated OA (FL-OA). Results: The formulated positions of OA were 0-155 ¡À 5 ¦Ìm for 2-MN and 175 ¡À 4 ¨C 225 ¡À 5 ¦Ìm for 3-MN. The administered doses of OA were 2.2 ¡À 0.1 ¦Ìg, 12.0 ¡À 0.2 ¦Ìg and 22.0 ¡À 0.2 ¦Ìg for 2-MN, 1.8 ¡À 0.2 ¦Ìg, 12.6 ¡À 0.7 ¦Ìg, and 20.4 ¡À 0.3 ¦Ìg for 3-MN, 10 ¦Ìg, 100 ¦Ìg and 1000 ¦Ìg for sc injection. At 4 weeks after the first administration, 3-MN showed about 2.5-7.0 fold and 5.4 fold higher total Ig (G + A + M) antibody than 2-MN and sc injection of the OA solution. Conclusions: The 3-MN, which delivered OA to the epidermis, is a useful drug delivery system for transcutaneous antigen delivery.
%K Dissolving Microneedles (Mns)
%K Two-Layered Mns
%K Three-Layered Mns
%K Transcutaneous Vaccine
%K Ovalbumin
%K Fl-Ovalbumin (Fl-Oa)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=20598