%0 Journal Article
%T Estandarizaci¨®n de un m¨¦todo para la extracci¨®n de DNA a partir de muestras cl¨ªnicas parafinadas
%A Tiscornia
%A Mar¨ªa M
%A Cubilla
%A Marisa A
%A Lorenzati
%A Mar¨ªa A
%A Cariaga Mart¨ªnez
%A Ariel E
%A Zapata
%A Pedro D
%J Revista de ciencia y tecnolog£¿-a
%D 2010
%I Scientific Electronic Library Online
%X molecular epidemiology includes genotypic studies of different biological processes, including pathological diseases such as cancer. therefore, the paraffin-embedded clinical samples analysis is very important for diagnosis, prevention and therapy. previously, many problems associated with amplification of dna obtained from paraffin-embedded tissue samples were reported. this paper compares the capability of nine methodologies for dna extraction from paraffin-embedded clinical samples, amplifying it by pcr to analyze under-250-bp fragments corresponding to folate pathway enzymes polymorphisms. modification of "salting-out" method with concentrations of 0.5 m nacl and 2 % ctab detergent and proteinase k was the most suitable for amplifiable dna obtention of selected amplicons. all methods showed better amplification with longer cycles and lower annealing temperatures as compared to less fragmented dna samples.
%K paraffin-embedded samples
%K dna extraction.
%U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S1851-75872010000200010&lng=en&nrm=iso&tlng=en