%0 Journal Article
%T Kill the Killers: terapia con c¨¦lulas Natural Killer en pacientes pedi¨¢tricos con c¨¢ncer refractario
%A Valent¨ªn Quiroga
%A J.
%A Fern¨¢ndez Casanova
%A L.
%A G¨¦nesis Mart¨ªn
%A I.
%A Nu£¿ez Mart¨ªn
%A F.
%A P¨¦rez Mart¨ªnez
%A A.
%J Sanidad Militar
%D 2012
%I Ministerio de Defensa
%R 10.4321/S1887-85712012000300003
%X introduction: refractory solid tumours lead children deaths. to change this statement, new treatments should be developed. natural killer cells constitute the first line of defence against tumour cells. we propose a new strategy for antitumor cell therapy in children with refractory solid malignancies: il-15 stimulated nk cells. patients and methods: 22 paediatric patients suffering refractory solid tumours participate in this study. we compare nk cell subsets and k562 cytotoxicity in patients and sex-age pair's healthy controls. we use multiparametric flow and time-resolved fluorescent, respectively. in immunocompromised mice we developed an aggressive human metastatic neuroblastoma model and il-15 stimulated nk cells rescue. results: patients had higher nk bright cells subset and lower nk cytotoxicity than healthy controls. il-15 stimulated nk cells improved nk cell cytotoxicity in vitro and decreased tumour burden in vivo. conclusions: il-15 stimulated nk cells may constitute a new cell therapy tool for paediatric refractory tumours.
%K natural killer cells (nk)
%K interleucina 15 (il-15)
%K refractory solid tumours.
%U http://scielo.isciii.es/scielo.php?script=sci_abstract&pid=S1887-85712012000300003&lng=en&nrm=iso&tlng=en