%0 Journal Article
%T Oxygen limitations to grow Azadirachta indica cell culture in shake flasks
%A Orozco-Sanchez
%A F.
%A Sep¨²lveda-Jim¨¦nez
%A G.
%A Trejo-Tapia
%A G.
%A Zamilpa
%A A.
%A Rodr¨ªguez-Monroy
%A M.
%J Revista mexicana de ingenier¨ªa qu¨ªmica
%D 2011
%I Universidad Aut¨®noma Metropolitana Unidad Iztapalapa
%X it was evaluated the growth of azadirachta indica cell suspension in different conditions of oxygen delivery in erlenmeyer shake flask. oxygen transfer rate (otr, kg o2 m-3 day-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). a. indica cells growing during 6 weeks of subculture showed that lower otr reduced cell viability, the ph of broth medium, and azadirachtins production. while, higher otr induced the formation of aggregates. using a stirred tank bioreactor, it was determined that a. indica cells had an oxygen consumption of 0.100 kg o2 kg dw-1 day-1, a higher value than other plant cell cultures. these results show that otr generated in erlenmeyer s hake flasks is lower to oxygen uptake rate of a. indica cells and it is a limiting factor to grow this plant.
%K azadirachta indica cell culture
%K shake flask
%K oxygen transfer rate
%K oxygen transfer resistance
%K closures.
%U http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S1665-27382011000300001&lng=en&nrm=iso&tlng=en