%0 Journal Article
%T Refractive index matching applied to fecal smear clearing
%A Ferreira
%A Cl¨˘udio S.
%J Revista do Instituto de Medicina Tropical de S£żo Paulo
%D 2005
%I Instituto de Medicina Tropical
%R 10.1590/S0036-46652005000600007
%X thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. aiming at practicability, a new quantitative technique has been developed. to enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. to each slide a template-measured (38.5 mm3) fecal sample is transferred. thus, egg counts and sensitivity evaluations are easily made.
%K turbidity of fecal suspensions
%K thick fecal smears
%K refractive index matching.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652005000600007&lng=en&nrm=iso&tlng=en