%0 Journal Article
%T H antigen presence in an Ascaris lumbricoides extract
%A Ponce de Le¨®n
%A Patricia
%A Foresto
%A Patricia
%A Valverde
%A Juana
%J Revista do Instituto de Medicina Tropical de S£¿o Paulo
%D 2005
%I Instituto de Medicina Tropical
%R 10.1590/S0036-46652005000300008
%X previous experiences have demonstrated the same abo system and p system antigens in a. lumbricoides extracts and in their hosts. the aim was to show the behavior of an a. lumbricoides extract from an o group patient against monoclonal antibodies of different specificities. agglutination inhibition tests were carried out facing the extract against monoclonal antibodies (anti a 2.23; anti b 2.54; anti b 2.62; anti ab 2.39 and anti h 2.72) in optimal concentrations. suspensions of o group fresh red cells were used as revealing system. the extract only inhibited the agglutination of anti h 2.72 with o erythrocytes. the semiquantitative agglutination inhibition test of the extract was made against two series of anti h 2.72 dilutions by using o group fresh red cells as revealing system. a difference of five dilutions between the titers of both series has been observed and the presence of h antigen in the extract has been significantly confirmed. the fact that the extract did not inhibit the agglutination against anti a, anti b and anti ab has corroborated our previous observations about absence of a and b epitopes in a. lumbricoides extracts from o group patients. the results of the preceding studies and this experience have demonstrated the membrane glycoconjugated importance in a. lumbricoides. they could be involved in molecular mimicry for this parasite.
%K h antigen
%K ascaris lumbricoides.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652005000300008&lng=en&nrm=iso&tlng=en