%0 Journal Article
%T Tnf-a production and apoptosis in hepatocytes after listeria monocytogenes and salmonella typhimurium invasion
%A Santos
%A Sania Alves Dos
%A Andrade J¨²nior
%A Dahir Ramos De
%A Andrade
%A Dahir Ramos De
%J Revista do Instituto de Medicina Tropical de S£¿o Paulo
%D 2011
%I Instituto de Medicina Tropical
%R 10.1590/S0036-46652011000200009
%X invasion of hepatocytes by listeria monocytogenes (lm) and salmonella typhimurium (st) can stimulate tumor necrosis factor alpha (tnf-¦Á) release and induce apoptosis. in this study, we compared the behavior of hepatocytes invaded by three l. monocytogenes serotypes (lm-4a, lm-4b and lm-1/2a) and by st to understand which bacterium is more effective in the infectious process. we quantified tnf-¦Á release by elisa, apoptosis rates by annexin v (early apoptosis) and tunel (late apoptosis) techniques. the cell morphology was studied too. tnf-¦Á release rate was highest in st-invaded hepatocytes. st and lm-1/2a induced the highest apoptosis production rates evaluated by tunel. lm-4b produced the highest apoptosis rate measured by annexin. invaded hepatocytes presented various morphological alterations. overall, lm-4b and lm-1/2a proved to be the most efficient at cell invasion, although st adapted faster to the environment and induced earlier hepatocyte tnf-¦Á release.
%K listeria monocytogenes
%K salmonella typhimurium
%K hepatocytes
%K apoptosis
%K tumor necrosis factor-alpha.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0036-46652011000200009&lng=en&nrm=iso&tlng=en