%0 Journal Article
%T Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
%A Maia
%A Maria de Mascena Diniz
%A Morais
%A Marcia Maria Camargo de
%A Morais Jr.
%A Marcos Antonio de
%A Melo
%A Eduardo Henrique Magalh£¿es
%A Lima Filho
%A Jos¨¦ Luiz de
%J Revista de Microbiologia
%D 1999
%I Scientific Electronic Library Online
%R 10.1590/S0001-37141999000400003
%X a brazilian strain of fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. the fungus produced 10,500 u.l-1 of lipase after 72 hours of cultivation at 25oc in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. glucose (1% w/v) was found to inhibit the inductive effect of olive oil. peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. the optimum lipase activity was achieved at ph 8.6 and 30oc and a good enzyme stability (80% activity retention) was observed at ph ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oc. lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.
%K enzyme kinetics
%K ester hydrolysis
%K extracellular lipase
%K fusarium solani.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0001-37141999000400003&lng=en&nrm=iso&tlng=en