%0 Journal Article
%T In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity
%A Santos
%A Lilian da Silva
%A Torres
%A Ros¨¢lia Morais
%A Machado-de-Assis
%A Girley Francisco
%A Bahia
%A Maria Terezinha
%A Martins
%A Helen Rodrigues
%A Teixeira-Carvalho
%A Andr¨¦a
%A Coelho-dos-Reis
%A Jordana Grazziela Alves
%A Albajar-Vi£¿as
%A Pedro
%A Martins-Filho
%A Olindo Assis
%A Lana
%A Marta de
%J Revista da Sociedade Brasileira de Medicina Tropical
%D 2012
%I Sociedade Brasileira de Medicina Tropical - SBMT
%R 10.1590/S0037-86822012000100008
%X introduction: the goal was to develop an in-house serological method with high specificity and sensitivity for diagnosis and monitoring of chagas disease morbidity. methods: with this purpose, the reactivities of anti-t. cruzi igg and subclasses were tested in successive serum dilutions of patients from berilo municipality, jequitinhonha valley, minas gerais, brazil. the performance of the in-house elisa was also evaluated in samples from other relevant infectious diseases, including hiv, hepatitis c (hcv), syphilis (syp), visceral leishmaniasis (vl), and american tegumentary leishmaniasis (atl), and noninfected controls (ni). further analysis was performed to evaluate the applicability of this in-house methodology for monitoring chagas disease morbidity into three groups of patients: indeterminate (ind), cardiac (card), and digestive/mixed (dig/mix), based on their clinical status. results: the analysis of total igg reactivity at serum dilution 1:40 was an excellent approach to chagas disease diagnosis (100% sensitivity and specificity). the analysis of igg subclasses showed cross-reactivity, mainly with ni, vl, and atl, at all selected serum dilutions. based on the data analysis, the ind group displayed higher igg3 levels and the dig/mix group presented higher levels of total igg as compared with the ind and card groups. conclusions: these findings demonstrated that methodology presents promising applicability in the analysis of anti-t. cruzi igg reactivity for the differential diagnosis and evaluation of chagas disease morbidity.
%K in-house elisa
%K igg subclasses
%K differential diagnosis
%K chagas disease morbidity.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0037-86822012000100008&lng=en&nrm=iso&tlng=en