%0 Journal Article
%T Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis
%A Silva
%A Jo£¿o Guilherme Lino da
%A Silva
%A Thiago Miranda da
%A Peloso
%A Eduardo de Figueiredo
%A Machado-Coelho
%A George Luiz Lins
%A Mayrink
%A Wilson
%A Ariosa
%A Mar¨ªlia Caixeta Franco
%A Faria e Silva
%A Paulo M¨¢rcio de
%A Marques
%A Marcos Jos¨¦
%J Revista da Sociedade Brasileira de Medicina Tropical
%D 2012
%I Sociedade Brasileira de Medicina Tropical - SBMT
%R 10.1590/S0037-86822012000200023
%X introduction: the study analyzed positivity of polymerase chain reaction (pcr) on detection of dna from leishmania in patients' samples. methods: extracted dna was submitted to l150/l152, 13y/13z, and seminested pcr (snpcr). results: results were evidenced by bands of approximately 120, 720, and 670 bp for l150/l152, 13y/13z, and snpcr, respectively. l150/l152, 13y/13z, and snpcr positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed. conclusions: preliminary results showed that these assays, mainly l150/l152 and snpcr, can detect leishmania dna and carry potential on laboratory diagnosis of leishmaniasis.
%K leishmania
%K pcr
%K diagnosis.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0037-86822012000200023&lng=en&nrm=iso&tlng=en