%0 Journal Article
%T Reemergence of yellow fever: detection of transmission in the State of S£żo Paulo, Brazil, 2008
%A Moreno
%A Eduardo Stramandinoli
%A Rocco
%A Iray Maria
%A Bergo
%A Eduardo Sterlino
%A Brasil
%A Roosecelis Araujo
%A Siciliano
%A Melissa Mascheratti
%A Suzuki
%A Akemi
%A Silveira
%A Vivian Regina
%A Bisordi
%A Ivani
%A Souza
%A Renato Pereira de
%J Revista da Sociedade Brasileira de Medicina Tropical
%D 2011
%I Sociedade Brasileira de Medicina Tropical - SBMT
%R 10.1590/S0037-86822011005000041
%X introduction: following yellow fever virus (yfv) isolation in monkeys from the s£żo jos¨Ĥ do rio preto region and two fatal human autochthonous cases from the ribeir£żo preto region, state of s£żo paulo, brazil, two expeditions for entomological research and eco-epidemiological evaluation were conducted. methods: a total of 577 samples from humans, 108 from monkeys and 3,049 mosquitoes were analyzed by one or more methods: virus isolation, elisa-igm, rt-pcr, histopathology and immunohistochemical. results: of the 577 human samples, 531 were tested by elisa-igm, with 3 positives, and 235 were inoculated into mice and 199 in cell culture, resulting in one virus isolation. one sample was positive by histopathology and immunohistochemical. using rt-pcr, 25 samples were processed with 4 positive reactions. a total of 108 specimens of monkeys were examined, 108 were inoculated into mice and 45 in cell culture. four virus strains were isolated from alouattacaraya. a total of 931 mosquitoes were captured in sao jose do rio preto and 2,118 in ribeir£żo preto and separated into batches. a single isolation of yfv was derived from a batch of 9 mosquitoes psorophoraferox, collected in urup¨şs, ribeir£żo preto region. a serological survey was conducted with 128 samples from the municipalities of s£żo carlos, rinc£żo and ribeir£żo preto and 10 samples from contacts of patients from ribeir£żo preto. all samples were negative by elisa-igm for yfv. conclusions: the results confirm the circulation of yellow fever, even though sporadic, in the sao paulo state and reinforce the importance of vaccination against yellow fever in areas considered at risk.
%K yellow fever
%K flavivirus
%K epizooties
%K entomological surveillance.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0037-86822011000300005&lng=en&nrm=iso&tlng=en