%0 Journal Article
%T Comparaci¨®n de los m¨¦todos de preservaci¨®n de espec¨Şmenes Anopheles (Diptera: Culicidae) para la extracci¨®n de ADN
%A Castano Sep¨˛lveda
%A Tatiana
%A Sanchez Zapata
%A Paola
%A Viana Guerra
%A Lorena
%A Correa Ochoa
%A Margarita
%A Gutierrez Builes
%A Lina
%A Zapata Tamayo
%A Mario
%J Revista Salud Uninorte
%D 2011
%I Universidad del Norte
%X abstract introduction: various methodologies have been reported for long term specimen preservation, which protect the dna from degradation allowing its posterior analysis in molecular studies. however, the effectiveness of the preservation methods may vary among insect groups; therefore, it is convenient to determine the preservation method to be used with a particular group before performing molecular studies with a large number of specimens. objetives: an experimental comparative study was conducted to evaluate different preservation treatments for anopheles albimanus, to know their utility in conserving the quantity and quality of dna to be used in subsequent molecular studies. methods: dna was extracted from each specimen, it was then quantified by spectrophotometer and the internal transcribed spacer 2 (its2) was amplified by pcr. results: its2 amplification was obtained in all specimens without previous preservation, in 60% of those preserved in s¨Şlica gel and in 20% of those preserved at -80ˇăc and in 70% ethanol. a significant difference (p<0.05) was found between amplification proportions. amplification was not obtained in specimens preserved frozen at -20ˇăc and not correlation by logistic regression was observed between the do260/do280 index and dna concentration of specimens presenting its2 amplification. conclusions: the data suggest that keeping specimens in silica gel or frozen at -80ˇăc may be the best conditions to preserve anopheles specimens for subsequent molecular studies.
%K anopheles
%K malaria
%K dna
%K samples preservation
%K polymerase chain reaction.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0120-55522011000100005&lng=en&nrm=iso&tlng=en