%0 Journal Article
%T Capacidad de los laboratorios nacionales de referencia en Latinoam¨¦rica para detectar mecanismos de resistencia emergentes
%A Corso
%A Alejandra
%A Guerriero
%A Leonor
%A Paster¨¢n
%A Fernando
%A Ceriana
%A Paola
%A Callejo
%A Raquel
%A Prieto
%A M¨®nica
%A Tuduri
%A Ezequiel
%A Lopardo
%A Horacio
%A Vay
%A Carlos
%A Smayevsky
%A Jorgelina
%A Tokumoto
%A Marta
%A ¨¢lvarez
%A Jorge Matheu
%A Pardo
%A Pilar Ram¨®n
%A Galas
%A Marcelo
%A 
%J Revista Panamericana de Salud P¨²blica
%D 2011
%I Organizaci¨®n Panamericana de la Salud
%R 10.1590/S1020-49892011001200021
%X objective: to evaluate the capability of 17 national reference laboratories participating in the latin american quality control program in bacteriology and antibiotic resistance (la-eqas) to detect emerging resistance mechanisms- namely: resistance of enterobacteria to carbapenems due to the presence of klebsiella pneumoniae carbapenemase (kpc) and metallo-beta-lactamase (mbl) type imp, and intermediate resistance of staphylococcus aureus isolates to vancomycin (vancomycin-intermediate resistant s. aureus-visa). methods: the following three isolates were sent to the 17 participating la-eqas laboratories: kpc -producing klebsiella pneumoniae paho-161, imp-producing enterobacter cloacae paho-166, and s. aureus paho-165 with intermediate resistance to vancomycin. performance of each of the following operations was evaluated: interpretation of sensitivity tests, detection of the resistance mechanism, and assessment of either inhibition halo size (disk diffusion method) or minimum inhibitory concentration (mic). results: concordance in the detection of resistance mechanisms was 76.4%, 73.3%, and 66.7% for the k. pneumoniae paho-161, e. cloacae paho-166, and s. aureus paho-165 strains, respectively. concordance between the inhibition areas observed by the participating laboratories and the ranges established by the coordinating laboratory was acceptable for all three isolates, at 90.8%, 92.8%, and 88.9%, respectively. conclusions: overall concordance in on the detection of kpc, mbl, and visa resistance mechanisms was 72.1%. we consider the national reference laboratories in latin america capable of recognizing these emerging resistance mechanisms and expect that maximum levels of concordance will be reached in the future.
%K quality control
%K drug resistance
%K microbial
%K enterobacteriacae
%K klebsiella pneumoniae
%K staphylococcus aureus
%K drug and narcotic control
%K laboratory proficiency testing
%K latin america.
%U http://www.scielosp.org/scielo.php?script=sci_abstract&pid=S1020-49892011001200021&lng=en&nrm=iso&tlng=en