%0 Journal Article
%T Spleen-Resident CD4+ and CD4£¿ CD8¦Á£¿ Dendritic Cell Subsets Differ in Their Ability to Prime Invariant Natural Killer T Lymphocytes
%A Emilie Bialecki
%A Elodie Macho Fernandez
%A Stoyan Ivanov
%A Christophe Paget
%A Josette Fontaine
%A Fabien Rodriguez
%A Luc Lebeau
%A Christophe Ehret
%A Benoit Frisch
%A Fran£¿ois Trottein
%A Christelle Faveeuw
%J PLOS ONE
%D 2011
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0026919
%X One important function of conventional dendritic cells (cDC) is their high capacity to capture, process and present Ag to T lymphocytes. Mouse splenic cDC subtypes, including CD8¦Á+ and CD8¦Á£¿ cDC, are not identical in their Ag presenting and T cell priming functions. Surprisingly, few studies have reported functional differences between CD4£¿ and CD4+ CD8¦Á£¿ cDC subsets. We show that, when loaded in vitro with OVA peptide or whole protein, and in steady-state conditions, splenic CD4£¿ and CD4+ cDC are equivalent in their capacity to prime and direct CD4+ and CD8+ T cell differentiation. In contrast, in response to ¦Á-galactosylceramide (¦Á-GalCer), CD4£¿ and CD4+ cDC differentially activate invariant Natural Killer T (iNKT) cells, a population of lipid-reactive non-conventional T lymphocytes. Both cDC subsets equally take up ¦Á-GalCer in vitro and in vivo to stimulate the iNKT hybridoma DN32.D3, the activation of which depends solely on TCR triggering. On the other hand, and relative to their CD4+ counterparts, CD4£¿ cDC more efficiently stimulate primary iNKT cells, a phenomenon likely due to differential production of co-factors (including IL-12) by cDC. Our data reveal a novel functional difference between splenic CD4+ and CD4£¿ cDC subsets that may be important in immune responses.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026919