%0 Journal Article
%T Evaluaci車n de un conjugado anti-IgG de rat車n-fluoresce赤na mediante t谷cnicas de inmunofluorescencia indirecta y citometr赤a de flujo
%A VILASECA
%A JUAN CARLOS
%A P谷REZ
%A LILIANA
%A SAV車N
%A CLARA
%A CHAC車N
%A DANAY
%A OROPESA FERN芍NDEZ
%A SUSET
%A RODR赤GUEZ
%A HERMIS
%A GOYENECHEA
%A 芍NGEL
%A OTERO
%A ANSELMO
%J Revista Cubana de Medicina Tropical
%D 1997
%I Centro Nacional de Informaci車n de Ciencias M谷dicas
%X an immunoglobulin g of mouse was purified from sera by affinity chromatography in protein a. the rabbits whose sera were able to recognize the antigen injected by double immunodiffusion were immunized with this preparation. the antibodies were precipatated from the rabbit's serum and purified by ion exchange chromatography. this preparation was conjugated to fluorescin isothiocyanate according to the conventional technique. the conjugated obtained was evaluated with the reference strains of parainfluenza virus 1, 2, 3; adenovirus; respiratory syncytial virus; and influenza virus a and b, by an indirect immunofluorescence technique and hiv positive samples by flow citometry. specific monoclonal antibodies were used in both cases. clinical specimens of patients with acute respiratory infection were evaluated.
%K igg [isolation & purification]
%K flow citometry [methods]
%K fluorescent antibody technique
%K indirect [methods]
%K mice [blood]
%K chromatography
%K affinity
%K chromatography
%K affinity punctures
%K staphylococcal protein a.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07601997000200009&lng=en&nrm=iso&tlng=en