%0 Journal Article
%T Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples
%A Vitale
%A Maria
%A Vitale
%A Fabrizio
%A Reale
%A Stefano
%A Vesco
%A Gesualdo
%A Curr¨°
%A Vittoria
%A Caracappa
%A Santo
%J Revista Cubana de Medicina Tropical
%D 2005
%I Centro Nacional de Informaci¨®n de Ciencias M¨¦dicas
%X it was carried out a comparison on two reference leptospira strains dna between different extraction methods and two polymerase chain reaction protocol. the dna was quantified and serial dilutions were tested by polymerase chain reaction. the results showed difference in terms of recovery and sensitivity between this methods.
%K leptospira
%K dna
%K extraction
%K pcr.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0375-07602005000100012&lng=en&nrm=iso&tlng=en