%0 Journal Article
%T SWI/SNF-Like Chromatin Remodeling Factor Fun30 Supports Point Centromere Function in S. cerevisiae
%A Micka£żl Durand-Dubief equal contributor
%A William Ryan Will equal contributor
%A Edoardo Petrini equal contributor
%A Delphine Theodorou
%A Rachael R. Harris
%A Margaret R. Crawford
%A Konrad Paszkiewicz
%A Felix Krueger
%A Rosa Maria Correra
%A Anna T. Vetter
%A J. Ross Miller
%A Nicholas A. Kent
%A Patrick Varga-Weisz
%J PLOS Genetics
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pgen.1002974
%X Budding yeast centromeres are sequence-defined point centromeres and are, unlike in many other organisms, not embedded in heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor conserved in humans, promotes point centromere function through the formation of correct chromatin architecture at centromeres. Our determination of the genome-wide binding and nucleosome positioning properties of Fun30 shows that this enzyme is consistently enriched over centromeres and that a majority of CENs show Fun30-dependent changes in flanking nucleosome position and/or CEN core micrococcal nuclease accessibility. Fun30 deletion leads to defects in histone variant Htz1 occupancy genome-wide, including at and around most centromeres. FUN30 genetically interacts with CSE4, coding for the centromere-specific variant of histone H3, and counteracts the detrimental effect of transcription through centromeres on chromosome segregation and suppresses transcriptional noise over centromere CEN3. Previous work has shown a requirement for fission yeast and mammalian homologs of Fun30 in heterochromatin assembly. As centromeres in budding yeast are not embedded in heterochromatin, our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture.
%U http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002974