%0 Journal Article
%T Targets of the Entamoeba histolytica Transcription Factor URE3-BP
%A Carol A. Gilchrist
%A Duza J. Baba
%A Yan Zhang
%A Oswald Crasta
%A Clive Evans
%A Elisabet Caler
%A Bruno W. S. Sobral
%A Christina B. Bousquet
%A Megan Leo
%A Ameilia Hochreiter
%A Sarah K. Connell
%A Barbara J. Mann
%A William A. Petri Jr
%J PLOS Neglected Tropical Diseases
%D 2008
%I Public Library of Science (PLoS)
%R 10.1371/journal.pntd.0000282
%X The Entamoeba histolytica transcription factor Upstream Regulatory Element 3-Binding Protein (URE3-BP) is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (Upstream Regulatory Element 3 (URE3)) in the promoter regions of hgl5 and fdx1. In this work, precise definition of the consensus URE3 element was performed by electrophoretic mobility shift assays (EMSA) using base-substituted oligonucleotides, and the consensus motif validated using episomal reporter constructs. Transcriptome profiling of a strain induced to produce a dominant-positive URE3-BP was then used to identify additional genes regulated by URE3-BP. Fifty modulated transcripts were identified, and of these the EMSA defined motif T[atg]T[tc][cg]T[at][tgc][tg] was found in over half of the promoters (54% p<0.0001). Fifteen of the URE3-BP regulated genes were potential membrane proteins, suggesting that one function of URE3-BP is to remodel the surface of E. histolytica in response to a calcium signal. Induction of URE3-BP leads to an increase in tranwell migration, suggesting a possible role in the regulation of cellular motility.
%U http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000282