%0 Journal Article
%T Evaluaci¨®n de diferentes crioprotectores para la crioconservaci¨®n de espermatozoides de yam¨² (Brycon amazonicus)
%A Cruz Casallas
%A Pablo E
%A Medina Robles
%A V¨ªctor M
%A Velasco Santamar¨ªa
%A Yohana M
%J Revista Colombiana de Ciencias Pecuarias
%D 2006
%I Universidad de Antioquia
%X the effect of dimethyl sulphoxide (dmso), ethylene glycol (etg), propylene glycol (ppg) and methanol (met) in yam¨² (brycon amazonicus - spix & agassiz, 1829) semen cryopreservation was evaluated with the aim of determining the post-thawing quality and in vitro fertility. for this purpose, sexually mature yam¨² females and males were induced to promote ovulation and spermation with carp pituitary extract. the semen obtained was evaluated and diluted 1:4 in a solution with 12% egg yolk and 5.5% glucose. different concentrations (3.75, 5, 7.5, 10, 11.25 and 15%) for each one of the 4 cryoprotectants were used for a total of 24 treatments. the diluted semen was frozen into 0.5 ml straws by liquid nitrogen vapours (ln) during 30 minutes and then transferred to a container with ln until be assayed. the straws (n= 6) corresponding to each treatment were thawed at 35 ¡ãc for 60 seconds and evaluated to select the best concentration for each cryoprotector for the fertility test. 5% dmso, 3.75% et, 3.75% ppg and 3.75% met treatments presented the highest motility percentages (mp) and activation time (at) being 5% dmso the best (mp 76 ¡À 2.4; at 88.2 ¡À 6.1). on the other hand, 3.75% ppg and 3.75% met treatments showed the highest fertility percentages (50 ¡À 1.4 and 47.8 ¡À 1.4, respectively); however, these percentages were significantly lower (p<0.05) than these obtained with fresh semen (64.7 ¡À 1.7). in general, it was observed that the increase in the cryoprotector concentration decreased semen quality post-thawing, and there was not direct relationship between sperm motility and fertility
%K cryoprotectant
%K fertilization
%K seminal quality
%K spermatozoa.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0120-06902006000200006&lng=en&nrm=iso&tlng=en