%0 Journal Article
%T New Method for Optimization and Simultaneous  Determination of Sparfloxacin and Non Steroidal Anti-Inflammatory Drugs: Its <i>In-Vitro</i> Application
%A Somia Gul
%A Najma Sultana
%A Muhammad Saeed Arayne
%A Sana Shamim
%A Mahwish Akhtar
%J American Journal of Analytical Chemistry
%P 328-337
%@ 2156-8278
%D 2012
%I Scientific Research Publishing
%R 10.4236/ajac.2012.34045
%X A simple reversed phase HPLC method was developed and validated for the simultaneous determination of sparfloxacin (SPFX), diclofenac sodium, meloxicam, ibuprofen, flurbiprofen, naproxen and mefenemic acid in a relatively short time with high linearity in bulk material, pharmaceutical formulations and human serum. Purospher STAR C<sub>18</sub> (250 ℅ 4.6 mm, 5 米m) column was utilized with mobile phase, methanol and water (90:10, v/v pH 2.70 adjusted by phosphoric acid), was delivered at a flow rate of 1.5 mL.min<sup>每1</sup>. Eluent was monitored using UV detector at 240 nm. The proposed method is specific, accurate (98.42% - 102.75%), precise (intra-day and inter-day variation 0.011% - 1.85%) and linear (R2 &gt; 0.999) with in the desired range 0.15 - 40 米g.mL<sup>每1</sup> and the detection and quantification limit was 1.19E+08 每 0.150 米g.mL<sup>每1</sup> and 3.62E+08 每 0.4574 米g.mL<sup>每1</sup> respectively for SPFX and NSAIDs. The analysis of variance (ANOVA) and student＊s t-test were applied to verify the results. The anticipated method is applicable to routine analysis of SPFX and NSAIDs in pharmaceutical formulations as well as in human serum samples. It has also applied on interaction of SPFX with NSAIDs.
%K Sparfloxacin
%K NSAIDs
%K HPLC
%K Interactions
%K ANOVA
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=18439