%0 Journal Article
%T Apoptosis, Shape Change and Reactive Oxygen Species Generation in Human Neutrophils Exposed to Olanzapine, an Atypical Antipsychotic Drug
%A Vargas
%A F
%A Ch¨¢vez
%A V
%A P¨¦rez
%A K
%J Revista Colombiana de Ciencias Qu¨ªmico - Farmac¨¦uticas
%D 2009
%I Scientific Electronic Library Online
%X the effects of olanzapine (olz) on the viability and functioning of human polymorphonuclear cells (pmns) are clearly opposite to those previously reported for clozapine (clz). in fact, after 4- or 24-h-treatment with 20-50 ¦Ìm olz, a significant inhibition of the respiratory burst in pmns activated with opsonized zimosan or phorbol myristate acetate (pma) was observed, whereas the burst provoked by formyl-methionyl-leucyl-phenylalanine (fmlp) was only inhibited at 50 ¦Ìm olz. under the same conditions, spontaneous apoptosis was accelerated at 20-50 ¦Ìm olz, while the exogenous addition of h2o2 resulted in the pmn apoptosis being dose-dependently inhibited by olz in the entire range of concentrations. however, when h2o2 was intracellularly generated by treatment with pma, the induced apoptosis was only decreased at 2 ¦Ìm olz. absorbance scans revealed that olz is able to react with equimolar quantities of either h2o2 or hocl. these results suggest that olz inhibits both ros-induced pmn apoptosis and respiratory burst due to extracellular scavenging of released ros.
%K olanzapine
%K neutrophils
%K apoptosis
%K respiratory burst
%K shape change
%K oxidative stress.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0034-74182009000100001&lng=en&nrm=iso&tlng=en