%0 Journal Article
%T Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars
%A EN¨¦AS-FILHO
%A JOAQUIM
%A BARBOSA
%A GISLAINY KARLA DA COSTA
%A SUD¨¦RIO
%A FABR¨ªCIO BONFIM
%A PRISCO
%A JOS¨¦ TARQU¨ªNIO
%A GOMES-FILHO
%A EN¨¦AS
%J Revista Brasileira de Fisiologia Vegetal
%D 2001
%I Scientific Electronic Library Online
%R 10.1590/S0103-31312001000300001
%X three isoforms of b-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of vita 3 and vita 5 cowpea [vigna unguiculata (l.) walp.] cultivars differing in water and salt stress tolerance. the purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through deae-sephadex and affinity chromatography through lactosyl-sepharose columns. the three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60oc), identical thermal stability up to 50¡ãc, and similar ph optima (3-4). however, they differed from each other in sensitivity towards metal ions and certain chemical agents presents in the assay medium. the results have shown that the observed differences in b-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance.
%K cotyledons
%K isozymes
%K vigna unguiculata
%K vita 3 and vita 5 cultivars
%K salt tolerance.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-31312001000300001&lng=en&nrm=iso&tlng=en