%0 Journal Article
%T Influ¨ºncia do h¨¢bito de fumar na excre£¿£¿o urin¨¢ria do ¨¢cido trans, trans-muc£¿nico
%A Menezes
%A Maico de
%A Balb£¿o
%A Marina Salviato
%A Siqueira
%A Maria Elisa Pereira Bastos de
%A Martins
%A Isarita
%J Revista Brasileira de Ci¨ºncias Farmac¨ºuticas
%D 2008
%I Scientific Electronic Library Online
%R 10.1590/S1516-93322008000300016
%X tobacco smoking is the major individual benzene source for no occupationally exposed subjects. this solvent is a volatile carcinogen and can induce serious health problems. several biomarkers for benzene have been proposed, the most used is its metabolite trans,trans-muconic acid (ttma) in urine, a suitable indicator since it reflects the internal dose of low benzene exposure. the present study aimed to evaluate the influence of smoking on urinary ttma excretion. the analysis was performed by high pressure liquid chromatography in a reversed-phase column and uv detection, after extraction of ttam from urine using sax cartridges. the method was validated, showing linearity between 0.006 to 10 ¦Ìg ml-1 (r= 0.996), detection and quantification limits of 1.41 x 10-3 ¦Ìg ml-1 and 6.0 x 10-3 ¦Ìg ml-1 respectively, cvs from 1.79 to 2.91% (intra assay precision) and 3.53 to 18.37% (interassay precision). a significant positive correlation was observed between ttma excretion and smoking (p= 0.005388). however, the cigarette number smoked by day seems have no influence on the urinary ttma excretion.
%K trans
%K trans-muconic acid
%K benzene [urinary identification]
%K smoking [toxicity]
%K high perfomance liquid chromatography [quantitative analysis].
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-93322008000300016&lng=en&nrm=iso&tlng=en