%0 Journal Article
%T Determina£¿£¿o do ¨¢cido trans,trans-muc£¿nico em urina: valida£¿£¿o de um m¨¦todo anal¨ªtico por cromatografia l¨ªquida de alta efici¨ºncia
%A Martins
%A Isarita
%A Siqueira
%A Maria Elisa Pereira Bastos de
%J Revista Brasileira de Ci¨ºncias Farmac¨ºuticas
%D 2002
%I Scientific Electronic Library Online
%R 10.1590/S1516-93322002000200010
%X the main purpose of biological monitoring is to protect the workers's health, preventing the toxic effects that can occur from occupational exposure to chemicals. trans, trans-muconic acid (ttma), a benzene metabolite, has been recommended as a sensitive bioindicator in the biological monitoring of workers exposed to this solvent. this work was developed in order to validate a method for ttma analysis in urine aiming its application on biomonitoring activities. the chosen technique was the high pressure liquid chromatography with reverse-phase column, lichrosorb rp 18, and uv detection. a linear relationship (r2= 0.9943) was observed in the range from 0.2 to 5.0 mg/l. the detection and quantification limits were respectively 0.1 and 0.2 mg/l. the average recovery was 77.1% and the inaccuracy (bias) was 27.9%. precision, evaluated by variation coefficient, were 7.7% (intra-assay) and 10.6% (inter-assay). the ttma remained stable in the matrix during a period of six weeks for the 0.2 mg/l samples and fifteen weeks for the 2.0 mg/l samples, in both cases when stored at -20 oc. in 0.2, 2.0 and 5.0 spiked samples no significant differences were found when conserved at 4 oc for ten days. when using the method to analyse post shift samples from benzene handling workers, the mean and median results found were, respectively, 0.8 and 0.6 mg ttma/g creatinine.
%K trans
%K trans-muconic acid
%K liquid chromatography
%K benzene.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-93322002000200010&lng=en&nrm=iso&tlng=en