%0 Journal Article
%T Desenvolvimento de metodologia anal¨ªtica para determina£¿£¿o de colesterol em ra£¿£¿o para ruminantes atrav¨¦s de planejamento experimental fatorial
%A Bandeira
%A Cec¨ªlia Muller
%A Ferreira
%A Jos¨¦ Maria
%A Bragagnolo
%A Neura
%A Mariutti
%A Lilian Regina Barros
%J Qu¨ªmica Nova
%D 2008
%I Sociedade Brasileira de Qu¨ªmica
%R 10.1590/S0100-40422008000600027
%X a chromatographic method was developed for cholesterol determination in feed for ruminants using response surface methodology. among the five approaches of sample preparation methods tested, the saponification of the sample without heating presented less interference in the gas chromatography. the method presented a relative standard deviation (rsd) of 4.3%, recoveries between 84 and 87% and detection limit of 0.001 mg of cholesterol per g of feed.
%K gc
%K extraction
%K validation.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-40422008000600027&lng=en&nrm=iso&tlng=en