%0 Journal Article
%T Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles
%A Ara¨²jo
%A Valdevane R.
%A Silva
%A Cleidson M. Gomes da
%A Magalh£¿es
%A Deborah M.
%A Silva
%A Gerlane Modesto da
%A B¨¢o
%A S£¿nia N.
%A Silva
%A Jos¨¦ Roberto Viana
%A Figueiredo
%A Jos¨¦ Ricardo de
%A Rodrigues
%A Ana Paula R.
%J Pesquisa Veterin¨¢ria Brasileira
%D 2010
%I Col¨¦gio Brasileiro de Patologia Animal - CBPA
%R 10.1590/S0100-736X2010000400004
%X this study was conducted in order to verify the effect of different concentrations of bmp-7 in the in vitro survival and development of caprine preantral follicles. fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in minimum essential medium (mem+) supplemented with different concentrations of bmp-7 (1, 10, 50 or 100ng/ml). non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (tem). parameters such as follicular survival, activation and growth were evaluated. the results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of bmp-7 for 1 day. in addition, the concentration of 10ng/ml of bmp-7 significantly increases follicular diameter from day 1 to 7 of culture. there was no influence of the other concentrations of bmp-7 regarding to the follicular and oocyte diameter. ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml bmp-7. in conclusion, small concentrations of bmp-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.
%K ovary
%K preantral follicles
%K growth factor
%K bone morphogenetic protein-7
%K bmp-7
%K goats.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-736X2010000400004&lng=en&nrm=iso&tlng=en