%0 Journal Article
%T Depletion of Murine Intestinal Microbiota: Effects on Gut Mucosa and Epithelial Gene Expression
%A Dag Henrik Reikvam
%A Alexander Erofeev
%A Anders Sandvik
%A Vedrana Grcic
%A Frode Lars Jahnsen
%A Peter Gaustad
%A Kathy D. McCoy
%A Andrew J. Macpherson
%A Leonardo A. Meza-Zepeda
%A Finn-Eirik Johansen
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0017996
%X Inappropriate cross talk between mammals and their gut microbiota may trigger intestinal inflammation and drive extra-intestinal immune-mediated diseases. Epithelial cells constitute the interface between gut microbiota and host tissue, and may regulate host responses to commensal enteric bacteria. Gnotobiotic animals represent a powerful approach to study bacterial-host interaction but are not readily accessible to the wide scientific community. We aimed at refining a protocol that in a robust manner would deplete the cultivable intestinal microbiota of conventionally raised mice and that would prove to have significant biologic validity.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0017996