%0 Journal Article
%T Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
%A Freschi
%A Carla Roberta
%A Carvalho
%A Luiz Fernando de Oliveira e Silva
%A Oliveira
%A Celso Jos¨Ļ Bruno de
%J Brazilian Journal of Microbiology
%D 2005
%I Sociedade Brasileira de Microbiologia
%R 10.1590/S1517-83822005000400011
%X the aim of this study was to compare different dna-extraction methods and selective enrichment broths for their effectiveness to detect salmonella typhimurium in artificially inoculated swine feces samples (100 cfu/g) by polymerase chain reaction. after enrichment in rappaport-vassiliadis, selenite cystine or m¨šller-kauffmann tetrathionate, aliquots were used for dna extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. aliquots of extracted dna were then used as template in pcr. the selective enrichment broths had no effect on the efficiency of pcr when boiling-centrifugation and salting-out were used. on the other hand, phenol-chloroform was superior (p<0.05) when combined to rappaport-vassiliadis. considering cost and efficiency parameters, we encourage the use of m¨šller-kauffmann tetrathionate broth in combination with boiling-centrifugation dna-extraction procedure.
%K diagnosis
%K dna
%K feces
%K pcr
%K salmonella typhimurium.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1517-83822005000400011&lng=en&nrm=iso&tlng=en