%0 Journal Article
%T Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
%A Maria-Cristina Keightley
%A Judith E. Layton
%A John W. Hayman
%A Joan K. Heath
%A Graham J. Lieschke
%J PLOS ONE
%D 2011
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0023845
%X Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023845