%0 Journal Article
%T High frequency wheat regeneration from leaf tissue explants of regenerated plantlets
%A Huimin Yu
%A Wenchao Wang
%A Yanwen Wang
%A Bingkai Hou
%J Advances in Bioscience and Biotechnology
%P 46-50
%@ 2156-8502
%D 2012
%I Scientific Research Publishing
%R 10.4236/abb.2012.31008
%X The specificities of tissue culture of wheat greatly limit the use of chloroplast transformation technologies in this crop. One limitation in wheat tissue culture is that it is difficult to regenerate plantlets from leaf tissue explants of regenerated plantlets, resulting in difficulty in obtaining homoplastic plants via multiple rounds of antibiotic selection of chloroplast transformants. Thus, a repeated in <i>vitro</i> regeneration system from leaf tissues was studied in this research. Our results showed that 2 mm leaf basal segments of the 4 cm high leaves from regenerated plantlets can give the best callus induction at present study. The best callus induction medium was Murashige and Skoog (MS) basal medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 1 mg/L naphthalenacetic acid, which gave a callus induction rate of up to 87.2%. The optimal differentiation medium was MS basal medium supplemented with 10 mg/L silver nitrate and 1 mg/L 2,3,5-triiodobenzoic acid, which gave a regeneration rate up to 33.7% for the wheat lines tested. This is the first report showing that leaf basal segments of <i>in vitro</i> regenerated plantlets can be used for regeneration of wheat. The establishment of a repetitive regeneration system should pave the way for the development of chloroplast transformation and the plant regeneration systems starting from leaf material of i<i>n vitro</i> regenerated wheat and other cereal crops.
%K Wheat (&lt
%K i&gt
%K Triticum aestivum&lt
%K /i&gt
%K L.)
%K &lt
%K i&gt
%K In Vitro&lt
%K /i&gt
%K Repetitive Regeneration
%K Leaf Segment
%K Tissue Culture
%K Chloroplast Transformation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17192