%0 Journal Article
%T Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
%A Falco
%A J.R.P.
%A Mello
%A M.L.S.
%J Genetics and Molecular Biology
%D 1999
%I Sociedade Brasileira de Gen¨¦tica
%R 10.1590/S1415-47571999000200010
%X the critical electrolyte concentrations (cec) of sperm chromatin from animal species known or suspected to contain histone h1 variants were compared by examining the affinity of their dna-protein complexes for toluidine blue in the presence of mg2+. bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. the cec for rana catesbeiana and two sea urchin species were similar to that of histone h5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these dna-protein complexes. the cec for bees and the bumblebee, bombus atratus, showed no particular phylogenetic relationship. we concluded that the cec of histone h1-containing sperm cell chromatin is a useful indicator of variability in dna-protein complexes but is of little phylogenetic value.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47571999000200010&lng=en&nrm=iso&tlng=en