%0 Journal Article
%T Consequences of primer binding-sites polymorphisms on genotyping practice
%A Estefania M. Martins
%A Laura Vilarinho
%A Sofia Esteves
%A M¨®nica Lopes-Marques
%A Ant¨®nio Amorim
%A Lu¨ªsa Azevedo
%J Open Journal of Genetics
%P 15-17
%@ 2162-4461
%D 2011
%I Scientific Research Publishing
%R 10.4236/ojgen.2011.12004
%X Herein we investigated the effect of primer binding site polymorphisms in achieving correct genotyping when a mismatch occurs in distinct positions of the primer sequence. For that purpose primer sequences were designed in order to carry either allelic form at the 3¡¯ end and at 3 bp, 5 bp and 7 bp apart from the 3¡¯ end of an intronic polymorphism (rs2247836) observed in phenylalanine hydroxylase (<i>PAH</i>) gene. For one of the alleles annealing failure was obtained when the mismatch occurs at all the four primer-site locations. Primer sequences carrying the alternative SNP allele resulted to be less specific as the distance to the primer-3¡¯ end was increased. Altogether, these results revealthat effects in the extension of the annealing failure is allele and mismatch-position dependent.
%K Primer Binding-site
%K SNP
%K &lt
%K i&gt
%K PAH&lt
%K /i&gt
%K Gene
%K Molecular Genetics
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=7314