%0 Journal Article
%T A novel method for Isolating Nanocrystalline Cellulose from Eucalyptus Hardwood
%A Jinrong Zhang
%A Wenbo Zou
%J Journal of Analytical Sciences, Methods and Instrumentation
%P 51-62
%@ 2164-2753
%D 2019
%I Scientific Research Publishing
%R 10.4236/jasmi.2019.93006
%X
The objective of this work is to develop a new method for extracting nanocrystalline
cellulose (CNC) from eucalyptus. CNC was isolated from eucalyptus
using 6:4 (v/v) tetrahydrofuran/water. The chemical and crystalline
structure of the isolated CNC was characterized by zeta potential measurements,
Fourier Transform Infrared (FT-IR) and UV-Vis spectroscopies, and
X-ray powder diffractometry. The CNC morphology was characterized by
Transmission Electron Microscope (TEM) and particle size of CNC was determined
by the Dynamic Light Scattering (DLS) method. Cytotoxicity and
zebrafish-toxicity experiments were also performed on CNC. The yield of
CNC using the new extraction method was about 41%, and the extracted
CNC particles were about 20 nm in diameter and about 40 nm long, on average.
The zeta potential of the CNC was determined to be -56 eV and the CN
particles were uniformly distributed in a suspension of 0.5 wt%. The prepared
CNC was a type I polymorph with an intact crystal structure. Toxicity results
revealed that 0.5 wt% is a safe CNC concentration, and that 2.5 mg/mL CNC
is not teratogenic or lethal to zebrafish embryos or juveniles. The new extraction
method developed in this study is environmentally friendly, and easy to
operate with relatively low cost.
%K Nanocrystalline Cellulose
%K Structure Characterization
%K Cytotoxicity
%K Zebrafish
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=95042