%0 Journal Article %T A novel method for Isolating Nanocrystalline Cellulose from Eucalyptus Hardwood %A Jinrong Zhang %A Wenbo Zou %J Journal of Analytical Sciences, Methods and Instrumentation %P 51-62 %@ 2164-2753 %D 2019 %I Scientific Research Publishing %R 10.4236/jasmi.2019.93006 %X
The objective of this work is to develop a new method for extracting nanocrystalline cellulose (CNC) from eucalyptus. CNC was isolated from eucalyptus using 6:4 (v/v) tetrahydrofuran/water. The chemical and crystalline structure of the isolated CNC was characterized by zeta potential measurements, Fourier Transform Infrared (FT-IR) and UV-Vis spectroscopies, and X-ray powder diffractometry. The CNC morphology was characterized by Transmission Electron Microscope (TEM) and particle size of CNC was determined by the Dynamic Light Scattering (DLS) method. Cytotoxicity and zebrafish-toxicity experiments were also performed on CNC. The yield of CNC using the new extraction method was about 41%, and the extracted CNC particles were about 20 nm in diameter and about 40 nm long, on average. The zeta potential of the CNC was determined to be -56 eV and the CN particles were uniformly distributed in a suspension of 0.5 wt%. The prepared CNC was a type I polymorph with an intact crystal structure. Toxicity results revealed that 0.5 wt% is a safe CNC concentration, and that 2.5 mg/mL CNC is not teratogenic or lethal to zebrafish embryos or juveniles. The new extraction method developed in this study is environmentally friendly, and easy to operate with relatively low cost.
%K Nanocrystalline Cellulose %K Structure Characterization %K Cytotoxicity %K Zebrafish %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=95042