%0 Journal Article
%T Cloning and Expression Analysis of <i>TTG</i>1 Gene Related to <i>Rosa rugosa</i> Trichomes Formation
%A Yu Wang
%A Mingyuan Zhao
%A Zongda Xu
%A Lanyong Zhao
%A Xu Han
%J American Journal of Plant Sciences
%P 265-275
%@ 2158-2750
%D 2019
%I Scientific Research Publishing
%R 10.4236/ajps.2019.102020
%X The <i>TTG</i>1 transcription factor plays an important role in the formation of plant trichomes. Based on the <i>R. rugosa</i> transcriptome data, this study cloned a <i>R. rugosa TTG</i>1 gene, named <i>RrTTG</i>1, and carried out bioinformatics analysis and fluorescence quantitative analysis to explore the relationship between <i>TTG</i>1 gene and <i>R. rugosa</i> trichomes formation, in order to lay a good foundation to cultivate a thornless plant in the family <i>Rosaceae</i>. In this experiment, six hybrid cultivars of <i>R. rugosa</i> ˇ°Zizhiˇ±, <i>R. rugosa</i> ˇ°Xiziˇ±, <i>R. rugosa</i> ˇ°Tang fenˇ±, <i>R. rugosa</i> ˇ°Hun chunˇ±, <i>R. rugosa</i> ˇ°Zi long wo chiˇ± and <i>R. rugosa</i> ˇ°Tian e huangˇ± were used as experimental materials, and the cDNA full length of this gene was obtained by RT-PCR and RACE, and the full length of the cDNA was 1348 bp. After bioinformatics analysis, it is predicted that its molecular formula is C<sub>1723</sub>H<sub>2661</sub>N<sub>465</sub>O<sub>529</sub>S<sub>12</sub>, the molecular weight is 38.71 KB, and the isoelectric point is 5.00. Its instability index is 54.30, which belongs to unstable protein; and its hydrophilic amino acid distribution is relatively uniform, and the amount is larger than hydrophobic amino acid, which belongs to hydrophilic protein. Phylogenetic tree was constructed for the <i>TTG</i>1 gene. Evolutionary analysis indicated that <i>RrTTG</i>1 is closely related to the <i>TTG</i>1 protein of <i>Rosaceae</i> family, and has a close relationship with other families. The expression analysis showed that the expression of <i>RrTTG</i>1 protein was negatively correlated with the trichome content of <i>R. rugosa</i> stems and leaves. The expression levels of the three spiny varieties of <i>R. rugosa</i> ˇ°Hun chunˇ±, <i>R. rugosa</i> ˇ°Xiziˇ± and <i>R. rugosa</i> ˇ°Zi long wo chiˇ± were lower, and the expressions of the three less thorn varieties of <i>R. rugosa</i> ˇ°Zizhiˇ±, <i>R. rugosa</i> ˇ°Tian e huangˇ± and <i>R. rugosa</i> ˇ°Tang fenˇ± were higher. According to the above results, it was speculated that <i>RrTTG</i>1 is involved in the synthesis of <i>R. rugosa</i> trichomes and belongs to the negative regulation mechanism.
%K &lt
%K i&gt
%K R. rugosa&lt
%K /i&gt
%K Trichome
%K &lt
%K i&gt
%K RrTTG&lt
%K /i&gt
%K 1
%K Gene Expression
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90443