%0 Journal Article %T Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate %A Shivani N. Patel %A Sandra D. Adams %A Lee H. Lee %J Advances in Bioscience and Biotechnology %P 679-690 %@ 2156-8502 %D 2018 %I Scientific Research Publishing %R 10.4236/abb.2018.912046 %X

Epigallocatechin gallate (EGCG), a green tea polyphenol possesses antioxidant, antibacterial, anticancer and antiviral properties. EGCG-Stearate (EGCG-S) is of interest for this study because of its stability and lipophilic properties. The chemical modification of EGCG-S increased its lipid solubility. Herpes simplex virus-1 (HSV-1), a member of the family Herpesviridae, and Alphaherpesvirinae subfamily is a leading cause of human viral diseases in the United States. In this study, 25 ¦ÌM, 50 ¦ÌM, 75 ¦ÌM, and 100 ¦ÌM of EGCG and EGCG-S were used to carry out cytotoxicity, cell viability and cell proliferation assays to determine the maximum non-cytotoxic concentrations on cultured A549 cells. The results suggested that 75 ¦ÌM of EGCG and EGCG-S is the appropriate concentration to further study the effect on the infection of HSV-1 in A549 cells. Infectivity, antiviral, and inverted microscopy assays were performed to study the effects of EGCG and EGCG-S on HSV-1 infection. An antiviral assay was performed using luminescence and it indicated that EGCG-S treated HSV-1 showed up to 90% inhibition. Confocal microscopy images further supported the inhibitory effects of 75 ¦ÌM EGCG-S on HSV-1 infection in A549 cells. The long-term goal of this research is to use EGCG-S as a possible novel topical therapeutic treatment to limit the spread of HSV-1 infections.

%K HSV-1 %K EGCG-Stearate %K EGCG %K Antiviral %K A549 Cells %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=89545