%0 Journal Article %T Improved Method for Determination of Raspberry Ketone in Fragrance Mist by HPLC-Fluorescence Analysis after Pre-Column Derivatization with 4-(<i>N,N</i>-Dimethylaminosulfonyl)-7-(<i>N</i>-chloroformylmethyl-<i>N</i>-methylamino)-2,1,3-benzoxadiazole %A Yasuhiko Higashi %J Journal of Analytical Sciences, Methods and Instrumentation %P 17-24 %@ 2164-2753 %D 2018 %I Scientific Research Publishing %R 10.4236/jasmi.2018.82002 %X

Raspberry ketone {RK, 4-(4-hydroxyphenyl)butan-2-one} is structurally resembles 4-(4-hydroxyphenyl)-2-butanol, which causes leukoderma on consumers¡¯ skin. Therefore, it is important to measure in cosmetics for quality assessment. Very recently, an HPLC-fluorescence method for determination of RK in a fragrance mist by pre-column derivatization with 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazine was established. However, the derivatization conditions (80&deg;C, 20 min) were severe. In this study, an improved pre-column derivatization with 4-(N,N-dimethylaminosulfonyl)-7-(N-chloro-formylmethyl-N-methylamino)-2,1,3-benzoxadiazole (DBD-COCl) is presented by HPLC-fluorescence method for determination of RK. The DBD-CO-RK derivative was eluted from a reversed-phase ODS column, and detected with excitation at 440 nm and emission at 543 nm. Derivatization was performed at room temperature for 3 min. The retention time of DBD-CO-RK derivative was 16.8 min. The standard curve was linear in the range of 0.05 to 2.5 ¦̀g/mL, with a correlation coefficient (r2) value of 0.9988. The lower limit of detection was 0.01 ¦̀g/mL (absolute amount of 0.3 pmol). The coefficients of variation were less than 10.0%. The content of RK in fragrance mist (1.00 mL) was 1.20 ¡À 0.08 mg (range, 1.10 to 1.31 mg, %K Raspberry Ketone %K High-Performance Liquid Chromatography %K 4-(< %K i> %K N %K N< %K /i> %K -Dimethylaminosulfonyl)-7-(< %K i> %K N< %K /i> %K -chloroformylmethyl-< %K i> %K N< %K /i> %K -methylamino)-2 %K 1 %K 3-benzoxadiazole %K Derivatization %K Fluo-rescence %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=85208