%0 Journal Article
%T Expression Vector Construction and Genetic Transformation of <i>Paeonia lactiflora</i> Gibberellin 20-Oxidase Gene
%A Lulu Han
%A Junjie Li
%A Jing Guo
%A Yan Ma
%A Xianfeng Guo
%J American Journal of Plant Sciences
%P 1525-1533
%@ 2158-2750
%D 2017
%I Scientific Research Publishing
%R 10.4236/ajps.2017.87105
%X GA20-oxidase (<i></i><i><i>GA</i></i>20<i></i><i><i>ox</i></i>) gene encodes a key
enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a <i></i><i><i>PlGA</i></i>20<i></i><i><i>ox</i><span \"=\"\"> </span></i><span \"=\"\">gene<i> </i>(GeneBank accession
number: KU886552) from </span><i></i><i><i>Paeonia lactiflora</i></i>. To further reveal
its function, we constructed an expression vector in the present study and then
transformed it into <i></i><i><i>Arabidopsis thaliana</i><span \"=\"\"> </span></i>plants by floral dip method. The transgenic plants
exhibited an early bolting, increased height and improved vegetative growth.
These results provided efficient vector tool and functional information of <i></i><i><i>PlGA</i></i>20<i></i><i><i>ox</i></i> for future gene
engineering in peony.
%K &lt
%K i&gt
%K PlGA&lt
%K /i&gt
%K 20&lt
%K i&gt
%K ox&lt
%K /i&gt
%K Expression Vector
%K &lt
%K i&gt
%K Arabidopsis thaliana&lt
%K /i&gt
%K Transformation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=76929