%0 Journal Article %T Effect of Adjunct Culture <i>Lactobacillus helveticus</i> (B02) on the Composition, Proteolysis, Free Amino Acids Release and Sensory Characteristics of Prato Cheese %A Nat¨¢lia Chinellato de Azambuja %A Izildinha Moreno %A Darlila Aparecida Gallina %A Leila Maria Spadoti %A Eliana Maria Pettirossi Motta %A Maria Teresa Bertolodo Pacheco %A Angela Lima Men¨ºses de Queiroz %A Adriane Elisabete Costa Antunes %J Food and Nutrition Sciences %P 512-525 %@ 2157-9458 %D 2017 %I Scientific Research Publishing %R 10.4236/fns.2017.85035 %X At semi-industrial scale it was obtained two cheeses, one with aromatic culture (CHN) and the other with also <i>L. helveticus</i> (CHN + LH). Proteolysis during ripening was evaluated using proteolytic indices, electrophoretic profile Urea-PAGE and release of free amino acids. Sensory evaluation was performed by acceptability test. It was observed a 5-fold increase in the fraction corresponding to <i>¦Á</i><sub>s1-I</sub>-casein in the CHN cheese and 6.5 times in the CHN + LH cheese and 7.2 and 8.2 increase respectively, for total amino acids. Prato cheese with the adjunct culture showed higher acceptability in terms of overall aroma and flavor. %K Amino Acids %K Cheese %K < %K i> %K L. helveticus< %K /i> %K Ripening %K Sensory Analysis %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=76192