%0 Journal Article
%T The Wnt Pathway Target Gene <i>CCND1</i> Changes Mitochondrial Localization and Decreases Mitochondrial Activity in Colorectal Cancer Cell Line SW480
%A Annica Vlad-Fiegen
%A Natalie Veronika Freytag
%A Susanne Dorn
%A Oliver M¨¹ller
%A Sonja Eberth
%J Journal of Biosciences and Medicines
%P 132-143
%@ 2327-509X
%D 2016
%I Scientific Research Publishing
%R 10.4236/jbm.2016.412017
%X Mutations leading to constitutive activation of the Wnt pathway and its target genes are frequently observed in cancer. The Wnt pathway promotes cell proliferation and increasing evidence supports its role also in cancer cell metabolism. This study aims to elucidate the role of the Wnt/¦Â-catenin target gene <em>CCND1</em> in these processes in colorectal cancer. We analyzed whether knock-down of <em>CCND1</em> affects cell cycle progression and energy metabolism in a colorectal cancer cell line. Down-regulation of <em>CCND1</em> led to retardation of the cell cycle. The proportion of cells in the G0 phase increased, while the amount of cells in the S- and G2/M phase decreased. Interestingly, knock-down of <em>CCND1</em> changed the perinuclear localization of mitochondria into a homogeneous distribution within the cytosol. In addition <em>CCND1</em> knock-down led to an increase of the intracellular ATP level indicating that cyclin D1 reduced mitochondrial activity. Our findings suggest that in addition to its role in cell cycle regulation, the Wnt target gene <em>CCND1</em> regulates mitochondrial localization and inhibits mitochondrial activity in colorectal cancer cells.
%K ATP
%K Cyclin D1
%K Colorectal Cancer
%K Mitochondria
%K Wnt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=72718