%0 Journal Article
%T 金果榄药材的UPLC指纹图谱研究<br>Study on UPLC Fingerprint of Tinospora sagittata and T. capillipes
%A 李扬华
%A 陈秋虹
%A 梁兰娜
%A 徐慧
%A 蒋艳芳
%J Advances in Analytical Chemistry
%P 79-85
%@ 2163-1565
%D 2016
%I Hans Publishing
%R 10.12677/AAC.2016.64012
%X <div style=\"text-align:justify;\">
	<span style=\"line-height:1.5;\">目的：建立金果榄药材的UPLC指纹图谱，为金果榄药材的质量控制提供依据。方法：采用超高效液相色谱法。色谱条件：色谱柱：Acquity UPLC<sup>？</sup>BEN C<sub>18</sub> (2.1 × 100 mm，1.7 μm)；流动相A：乙腈；流动相B：0.3%磷酸溶液；流速：0.25 ml/min；检测波长：225 nm；柱温：25℃。结果：建立了10批金果榄药材的指纹图谱。金果榄药材有7个共有峰，多数峰可以达到较好分离，具有较高的相似度。结论：建立的超高效液相指纹图谱有较好的精密度、重复性和稳定性，可作为金果榄质量评价参考。<br />
Objective: To establish UPLC fingerprint of Tinospora sagittata and T. capillipes which is expected to provide standards for the quality control and identification of the Chinese crude drug. Method: The UPLC method was used. Chromatography conditions were Acquity UPLC<sup>？</sup>BEN C<sub>18 </sub>(2.1 × 100 mm, 1.7 μm) column with gradient mobile phase of acetonitrile and 0.3% phosphate acid. UV detection wavelength was 225 nm, the flow rate was 0.25 ml/min and the column temperature was 25？C. Result: UPLC fingerprints of 10 samples of Tinospora sagittata and T. capillipes were established. 7 mutual peaks were selected as the fingerprint peaks in the samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 10 samples had higher simi-larities. Conclusion: The established UPLC fingerprint has desirable accuracy, repeatability and sta-bility, which can be used as reference for the quality control of Tinospora sagittata and T. capillipes.</span><span style=\"line-height:1.5;\"></span> 
</div>
%K 金果榄，UPLC，指纹图谱，质量控制 <br>Tinospora sagittata and T. capillipes
%K UPLC
%K Fingerprints
%K Quality Control
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=18749